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Coding
Part:BBa_K3629022:Design
Designed by: Arshia Mostoufi Group: iGEM20_Calgary (2020-10-12)
mCitrine codon optimized for Yarrowia lipolytica
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 682
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 647
Design Notes
Below is the codon-optimized sequence of Yarrowia lipolytica mChitrine for use in any expression construct. However, we have also made our own expression construct BBa_K3629026, designed to allow for optimal expression of mChitrine in Yarrowia lipolytica using the TEfin promoter BBa_K3629001 and the XPR2 terminator BBa_K3629004.
Source
Original Sequence obtained from https://www.fpbase.org/protein/mcitrine/
References
1. [online] https://www.fpbase.org/protein/mcitrine/ (Accessed October 26, 2020)